Immunofluorescence staining of A549 cells with CSB-PA748484HA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of A549 cells with CSB-PA748484HA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
HECW1 Antibody
CSB-PA748484HA01HU
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
ReactivityHuman
TargetHECW1
Overview
- SupplierCusabio
- Product NameHECW1 Antibody
- Delivery Days Customer20
- ApplicationsImmunoFluorescence, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID23072
- Target nameHECW1
- Target descriptionHECT, C2 and WW domain containing E3 ubiquitin protein ligase 1
- Target synonymsNEDL1, E3 ubiquitin-protein ligase HECW1, HECT type E3 ubiquitin ligase, HECT-type E3 ubiquitin transferase HECW1, NEDD4-like ubiquitin-protein ligase 1
- HostRabbit
- IsotypeIgG
- Protein IDQ76N89
- Protein NameE3 ubiquitin-protein ligase HECW1
- Scientific DescriptionE3 ubiquitin-protein ligase that mediates ubiquitination and subsequent degradation of DVL1. Also targets the mutant SOD1 protein involved in familial amyotrophic lateral sclerosis (FALS). Forms cytotoxic aggregates with DVL1, SSR3 and mutant SOD1 that lead to motor neuron death in FALS.
- ReactivityHuman
- Storage Instruction-20°C or -80°C
- UNSPSC41116161
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