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Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA868307HA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA868307HA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA868307HA01HU at dilution of 1:100

HELLS Antibody

CSB-PA868307HA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetHELLS
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Overview

  • Supplier
    Cusabio
  • Product Name
    HELLS Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3070
  • Target name
    HELLS
  • Target description
    helicase, lymphoid specific
  • Target synonyms
    ICF4, LSH, Nbla10143, PASG, SALNR, SMARCA6, lymphoid-specific helicase, SWI/SNF2-related, matrix-associated, actin-dependent regulator of chromatin, subfamily A, member 6, Senescence Associated Long Non-coding RNA, proliferation-associated SNF2-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NRZ9
  • Protein Name
    Lymphoid-specific helicase
  • Scientific Description
    Plays an essential role in normal development and survival. Involved in regulation of the expansion or survival of lymphoid cells. Required for de novo or maintenance DNA methylation. May control silencing of the imprinted CDKN1C gene through DNA methylation. May play a role in formation and organization of heterochromatin, implying a functional role in the regulation of transcription and mitosis (By similarity).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HELLS using anti-HELLS antibody (A04702-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: mouse thymus tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HELLS antigen affinity purified polyclonal antibody (Catalog # A04702-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HELLS at approximately 97 kDa. The expected band size for HELLS is at 97 kDa.
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