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ProductsBack/Hemoglobin zeta antibody [GT1544]

Hemoglobin zeta antibody [GT1544]

GTX633545
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetHBZ
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Overview

  • Supplier
    GeneTex
  • Product Name
    Hemoglobin zeta antibody [GT1544]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GT1544
  • Concentration
    1.54 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3050
  • Target name
    HBZ
  • Target description
    hemoglobin subunit zeta
  • Target synonyms
    HBAZ; HBZ1; HBZ-T1; hemoglobin subunit zeta; hemoglobin zeta chain; hemoglobin, zeta; zeta-globin
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP02008
  • Protein Name
    Hemoglobin subunit zeta
  • Scientific Description
    Zeta-globin is an alpha-like hemoglobin. The zeta-globin polypeptide is synthesized in the yolk sac of the early embryo, while alpha-globin is produced throughout fetal and adult life. The zeta-globin gene is a member of the human alpha-globin gene cluster that includes five functional genes and two pseudogenes. The order of genes is: 5 - zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 -alpha-1 - theta1 - 3. [provided by RefSeq, Nov 2009]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HBZ using anti-HBZ antibody (A03815-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat bone tissue lysates, Lane 2: mouse bone tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HBZ antigen affinity purified polyclonal antibody (A03815-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HBZ at approximately 14 kDa. The expected band size for HBZ is at 16 kDa.
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