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HEPACAM2 Polyclonal Antibody

BS-8417R
Bioss
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetHEPACAM2
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Overview

  • Supplier
    Bioss
  • Product Name
    HEPACAM2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID253012
  • Target name
    HEPACAM2
  • Target description
    HEPACAM family member 2
  • Target synonyms
    MIKI, HEPACAM family member 2, mitotic kinetics regulator
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDA8MVW5
  • Protein Name
    HEPACAM family member 2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of HEPACAM2 using anti-HEPACAM2 antibody (A12313-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HEPACAM2 antigen affinity purified polyclonal antibody (Catalog # A12313-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HEPACAM2 at approximately 65 kDa. The expected band size for HEPACAM2 is at 51 kDa.
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