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ProductsBack/Hepatocyte Nuclear Factor 1 Beta (HNF1b) Polyclonal Antibody

Hepatocyte Nuclear Factor 1 Beta (HNF1b) Polyclonal Antibody

CAU22162
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetHNF1B
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Overview

  • Supplier
    Biomatik
  • Product Name
    Hepatocyte Nuclear Factor 1 Beta (HNF1b) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6928
  • Target name
    HNF1B
  • Target description
    HNF1 homeobox B
  • Target synonyms
    ADTKD3, FJHN, HNF-1-beta, HNF-1B, HNF1beta, HNF2, HPC11, LF-B3, LFB3, MODY5, RCAD, T2D, TCF-2, TCF2, VHNF1, hepatocyte nuclear factor 1-beta, HNF1 beta A, homeoprotein LFB3, transcription factor 2, hepatic
  • Host
    Rabbit
  • Protein IDP35680
  • Protein Name
    Hepatocyte nuclear factor 1-beta
  • Scientific Description
    The Hepatocyte Nuclear Factor 1 Beta (HNF1b) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HNF1 beta using anti-HNF1 beta antibody (PB9597). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Liver Tissue Lysate at 50ug, Lane 2: Rat Kidney Tissue Lysate at 50ug, Lane 3: SW620 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HNF1 beta antigen affinity purified polyclonal antibody (Catalog # PB9597) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HNF1 beta at approximately 61 kDa. The expected band size for HNF1 beta is at 61 kDa.
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Immunohistochemistry analysis in human kidney and tonsil tissues using AMAb90733 antibody. Corresponding HNF1B RNA-seq data are presented for the same tissues.
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