Detection of endogenous erbB2 Whole cell extracts of serum starved, EGF-treated (15 min) tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab erbB2-24B5 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2A 549 Lane 3: SKOV3 Lane 4: OVCAR5 Lane 5: HaCaT Lane 6: PC3 Lane 7: HeLa Lane 8: HepG2
Her2 (ERBB2) (860-880) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 24B5]
AM00049PU-N
ApplicationsWestern Blot
Product group Antibodies
ReactivityCanine, Human, Mouse
TargetERBB2
Overview
- SupplierOriGene
- Product NameHer2 (ERBB2) (860-880) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 24B5]
- Delivery Days Customer14
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID24B5
- Gene ID2064
- Target nameERBB2
- Target descriptionerb-b2 receptor tyrosine kinase 2
- Target synonymsCD340; c-erb B2/neu protein; HER2; HER-2; HER-2/neu; herstatin; human epidermal growth factor receptor 2; metastatic lymph node gene 19 protein; MLN 19; NEU; neuro/glioblastoma derived oncogene homolog; neuroblastoma/glioblastoma derived oncogene homolog; NGL; proto-oncogene c-ErbB-2; proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog; VSCN2
- HostMouse
- IsotypeIgG1
- Protein IDP04626
- Protein NameReceptor tyrosine-protein kinase erbB-2
- Scientific DescriptionHer2 (ERBB2) (860-880) (incl. pos. control) mouse monoclonal antibody, clone 24B5, Purified
- ReactivityCanine, Human, Mouse
- UNSPSC12352203