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ErbB2 activation: Serum starved A549 cells were incubated with 10 ng/ml EGF for the indicated times. Whole cell lysates were prepared with lysis buffer V19 and separated by SDS-PAGE (ca 20.000 cells/lane). The immunoblot was probed with mab erbB2-19G5 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: Control Lane 2: 5 min EGF Lane 3: 15 min EGF Lane 4: 30 min EGF Lane 5: 1h EGF Lane 6; 2h EGF Lane 7: 4h EGF Lane 8: 8h EGF
ErbB2 activation: Serum starved A549 cells were incubated with 10 ng/ml EGF for the indicated times. Whole cell lysates were prepared with lysis buffer V19 and separated by SDS-PAGE (ca 20.000 cells/lane). The immunoblot was probed with mab erbB2-19G5 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: Control Lane 2: 5 min EGF Lane 3: 15 min EGF Lane 4: 30 min EGF Lane 5: 1h EGF Lane 6; 2h EGF Lane 7: 4h EGF Lane 8: 8h EGF
ErbB2 activation: Serum starved A549 cells were incubated with 10 ng/ml EGF for the indicated times. Whole cell lysates were prepared with lysis buffer V19 and separated by SDS-PAGE (ca 20.000 cells/lane). The immunoblot was probed with mab erbB2-19G5 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: Control Lane 2: 5 min EGF Lane 3: 15 min EGF Lane 4: 30 min EGF Lane 5: 1h EGF Lane 6; 2h EGF Lane 7: 4h EGF Lane 8: 8h EGF

Her2 (ERBB2) pTyr1112 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 19G5]

Research Use Only
AM00050PU-N
OriGene
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityCanine, Human, Mouse, Rat
TargetERBB2
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Her2 (ERBB2) pTyr1112 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 19G5]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    19G5
  • Gene ID2064
  • Target name
    ERBB2
  • Target description
    erb-b2 receptor tyrosine kinase 2
  • Target synonyms
    CD340; c-erb B2/neu protein; HER2; HER-2; HER-2/neu; herstatin; human epidermal growth factor receptor 2; metastatic lymph node gene 19 protein; MLN 19; NEU; neuro/glioblastoma derived oncogene homolog; neuroblastoma/glioblastoma derived oncogene homolog; NGL; proto-oncogene c-ErbB-2; proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog; VSCN2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP04626
  • Protein Name
    Receptor tyrosine-protein kinase erbB-2
  • Scientific Description
    Her2 (ERBB2) pTyr1112 (incl. pos. control) mouse monoclonal antibody, clone 19G5, Purified
  • Reactivity
    Canine, Human, Mouse, Rat
  • UNSPSC
    12352203