ErbB2 activation: Serum starved A549 cells were incubated with 10 ng/ml EGF for the indicated times. Whole cell lysates were prepared with lysis buffer V19 and separated by SDS-PAGE (ca 20.000 cells/lane). The immunoblot was probed with mab erbB2-19G5 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: Control Lane 2: 5 min EGF Lane 3: 15 min EGF Lane 4: 30 min EGF Lane 5: 1h EGF Lane 6; 2h EGF Lane 7: 4h EGF Lane 8: 8h EGF
Her2 (ERBB2) pTyr1112 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 19G5]
AM00050PU-N
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityCanine, Human, Mouse, Rat
TargetERBB2
Overview
- SupplierOriGene
- Product NameHer2 (ERBB2) pTyr1112 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 19G5]
- Delivery Days Customer14
- ApplicationsImmunoFluorescence, Western Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID19G5
- Gene ID2064
- Target nameERBB2
- Target descriptionerb-b2 receptor tyrosine kinase 2
- Target synonymsCD340; c-erb B2/neu protein; HER2; HER-2; HER-2/neu; herstatin; human epidermal growth factor receptor 2; metastatic lymph node gene 19 protein; MLN 19; NEU; neuro/glioblastoma derived oncogene homolog; neuroblastoma/glioblastoma derived oncogene homolog; NGL; proto-oncogene c-ErbB-2; proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog; VSCN2
- HostMouse
- IsotypeIgG1
- Protein IDP04626
- Protein NameReceptor tyrosine-protein kinase erbB-2
- Scientific DescriptionHer2 (ERBB2) pTyr1112 (incl. pos. control) mouse monoclonal antibody, clone 19G5, Purified
- ReactivityCanine, Human, Mouse, Rat
- UNSPSC12352203