erbB2 activation Serum starved OVCAR-5 cells were incubated with 10 ng/ml EGF for the indicated times. Whole cell lysates were prepared with lysis buffer V19 and separated by SDS-PAGE (ca 20.000 cells/lane). The immunoblot was probed with mab erbB2 - 6G7 (0.5 ug/ ml) for 1h at RTand developed by ECL (exp. time: 30 sec). lane 1: control; lane 2: 5 min EGF; lane 3: 15 min EGF; lane 4: 30 min EGF; lane 5: 1h EGF;lane 6; 2h EGF; lane 7: 4h EGF; lane 8: 8h EGF
Her2 (ERBB2) pTyr1248 Mouse Monoclonal Antibody [Clone ID: 6G7]
AM00053PU-N
Overview
- SupplierOriGene
- Product NameHer2 (ERBB2) pTyr1248 Mouse Monoclonal Antibody [Clone ID: 6G7]
- Delivery Days Customer14
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID6G7
- Gene ID2064
- Target nameERBB2
- Target descriptionerb-b2 receptor tyrosine kinase 2
- Target synonymsCD340; c-erb B2/neu protein; HER2; HER-2; HER-2/neu; herstatin; human epidermal growth factor receptor 2; metastatic lymph node gene 19 protein; MLN 19; NEU; neuro/glioblastoma derived oncogene homolog; neuroblastoma/glioblastoma derived oncogene homolog; NGL; proto-oncogene c-ErbB-2; proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog; VSCN2
- HostMouse
- IsotypeIgG1
- Protein IDP04626
- Protein NameReceptor tyrosine-protein kinase erbB-2
- Scientific DescriptionHer2 (ERBB2) pTyr1248 mouse monoclonal antibody, clone 6G7, Purified
- ReactivityHuman
- Storage Instruction-20°C,-80°C,2°C to 8°C
- UNSPSC12352203