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ProductsBack/Her2 (ERBB2) pTyr1248 Mouse Monoclonal Antibody [Clone ID: 6G7]
erbB2 activation Serum starved OVCAR-5 cells were incubated with 10 ng/ml EGF for the indicated times. Whole cell lysates were prepared with lysis buffer V19 and separated by SDS-PAGE (ca 20.000 cells/lane). The immunoblot was probed with mab erbB2 - 6G7 (0.5 ug/ ml) for 1h at RTand developed by ECL (exp. time: 30 sec). lane 1: control; lane 2: 5 min EGF; lane 3: 15 min EGF; lane 4: 30 min EGF; lane 5: 1h EGF;lane 6; 2h EGF; lane 7: 4h EGF; lane 8: 8h EGF
erbB2 activation Serum starved OVCAR-5 cells were incubated with 10 ng/ml EGF for the indicated times. Whole cell lysates were prepared with lysis buffer V19 and separated by SDS-PAGE (ca 20.000 cells/lane). The immunoblot was probed with mab erbB2 - 6G7 (0.5 ug/ ml) for 1h at RTand developed by ECL (exp. time: 30 sec). lane 1: control; lane 2: 5 min EGF; lane 3: 15 min EGF; lane 4: 30 min EGF; lane 5: 1h EGF;lane 6; 2h EGF; lane 7: 4h EGF; lane 8: 8h EGF
erbB2 activation Serum starved OVCAR-5 cells were incubated with 10 ng/ml EGF for the indicated times. Whole cell lysates were prepared with lysis buffer V19 and separated by SDS-PAGE (ca 20.000 cells/lane). The immunoblot was probed with mab erbB2 - 6G7 (0.5 ug/ ml) for 1h at RTand developed by ECL (exp. time: 30 sec). lane 1: control; lane 2: 5 min EGF; lane 3: 15 min EGF; lane 4: 30 min EGF; lane 5: 1h EGF;lane 6; 2h EGF; lane 7: 4h EGF; lane 8: 8h EGF

Her2 (ERBB2) pTyr1248 Mouse Monoclonal Antibody [Clone ID: 6G7]

AM00053PU-N
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetERBB2
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Her2 (ERBB2) pTyr1248 Mouse Monoclonal Antibody [Clone ID: 6G7]
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    6G7
  • Gene ID2064
  • Target name
    ERBB2
  • Target description
    erb-b2 receptor tyrosine kinase 2
  • Target synonyms
    CD340, HER-2, HER-2/neu, HER2, MLN 19, MLN-19, NEU, NGL, TKR1, VSCN2, c-ERB-2, c-ERB2, p185(erbB2), receptor tyrosine-protein kinase erbB-2, c-erb B2/neu protein, herstatin, human epidermal growth factor receptor 2, metastatic lymph node gene 19 protein, neuro/glioblastoma derived oncogene homolog, neuroblastoma/glioblastoma derived oncogene homolog, proto-oncogene Neu, proto-oncogene c-ErbB-2, tyrosine kinase-type cell surface receptor HER2, v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2, v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2, v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP04626
  • Protein Name
    Receptor tyrosine-protein kinase erbB-2
  • Scientific Description
    Her2 (ERBB2) pTyr1248 mouse monoclonal antibody, clone 6G7, Purified
  • Reactivity
    Human
  • Storage Instruction
    -20°C,-80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ErbB 2 using anti-ErbB 2 antibody (A00010-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ErbB 2 antigen affinity purified polyclonal antibody (Catalog # A00010-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ErbB 2 at approximately 185 kDa. The expected band size for ErbB 2 is at 138 kDa.
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