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HFE Monoclonal Antibody

CAB0937
Assay Genie
ReactivityHuman
Product group Antibodies
TargetHFE
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Overview

  • Supplier
    Assay Genie
  • Product Name
    HFE Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID3077
  • Target name
    HFE
  • Target description
    homeostatic iron regulator
  • Target synonyms
    HFE1, HH, HLA-H, MVCD7, TFQTL2, hereditary hemochromatosis protein, MHC class I-like protein HFE, hereditary hemochromatosis protein HLA-H, high Fe
  • Host
    Rabbit
  • Protein IDQ30201
  • Protein Name
    Hereditary hemochromatosis protein
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HFE using anti-HFE antibody (A00506-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA whole Cell lysates, Lane 2: HEPG2 whole Cell lysates, Lane 3: A431 whole Cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HFE antigen affinity purified polyclonal antibody (Catalog # A00506-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HFE at approximately 40KD. The expected band size for HFE is at 40KD.
Product group Antibodies
Boster Bio
Anti-HFE Antibody Picoband(r)A00506-1-CARRIER-FREE
ApplicationsWestern Blot
ReactivityHuman
TargetHFE
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Product group Antibodies
RayBiotech
Anti-HFE Antibody144-01310
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman, Mouse
TargetHFE
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HL-60 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with _x000D_ HFE/Hemochromatosis Polyclonal Antibody(bs-12335R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Product group Antibodies
References
Bioss
HFE Polyclonal AntibodyBS-12335R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Equine, Human, Mouse, Rat, Sheep
TargetHFE
  • SizePrice
Product group Antibodies
Cusabio
HFE AntibodyCSB-PA653744LA01HU
ApplicationsELISA
ReactivityHuman
TargetHFE
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LifeSpan BioSciences
HFE AntibodyLS-C331410
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ReactivityHuman, Mouse
TargetHFE
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Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.
Product group Antibodies
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Anti-HFE AntibodyHPA017276
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TargetHFE
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Anti-HFE AntibodyA29875
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Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with HFE antibody (GTX135988) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GeneTex
HFE antibodyGTX135988
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ReactivityHuman
TargetHFE
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