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ProductsBack/HGD antibody, Internal
WB analysis of HeLa cells using GTX45853 HGD antibody at 0.2-1microg/ml.
WB analysis of HeLa cells using GTX45853 HGD antibody at 0.2-1microg/ml.
WB analysis of HeLa cells using GTX45853 HGD antibody at 0.2-1microg/ml.

HGD antibody, Internal

GTX45853
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetHGD
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Overview

  • Supplier
    GeneTex
  • Product Name
    HGD antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3081
  • Target name
    HGD
  • Target description
    homogentisate 1,2-dioxygenase
  • Target synonyms
    AKU; HGO; homogentisate 1,2-dioxygenase; homogentisate oxidase; homogentisate oxygenase; homogentisic acid oxidase; homogentisicase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ93099
  • Protein Name
    Homogentisate 1,2-dioxygenase
  • Scientific Description
    This gene encodes the enzyme homogentisate 1,2 dioxygenase. This enzyme is involved in the catabolism of the amino acids tyrosine and phenylalanine. Mutations in this gene are the cause of the autosomal recessive metabolism disorder alkaptonuria.[provided by RefSeq, May 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HGD using anti-HGD antibody (A01909-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HGD antigen affinity purified polyclonal antibody (Catalog # A01909-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HGD at approximately 50KD. The expected band size for HGD is at 50KD.
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