Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HGF Polyclonal Antibody, Unconjugated (bs-1025R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HGF Polyclonal Antibody, Unconjugated (bs-1025R) at 1:400 overnight at 4°C, DAB staining.
HGF Polyclonal Antibody
BS-1025R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetHGF
Overview
- SupplierBioss
- Product NameHGF Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID3082
- Target nameHGF
- Target descriptionhepatocyte growth factor
- Target synonymsDFNB39, F-TCF, HGFB, HPTA, SF, hepatocyte growth factor, fibroblast-derived tumor cytotoxic factor, hepatocyte growth factor (hepapoietin A; scatter factor), hepatopoietin-A, lung fibroblast-derived mitogen
- HostRabbit
- IsotypeIgG
- Protein IDP14210
- Protein NameHepatocyte growth factor
- ReactivityBovine, Canine, Equine, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat, Sheep
- Storage Instruction-20°C
- UNSPSC41116161
References
- Potential biomarker of fibroblast growth factor 21 in valproic acid-treated livers. Xu X et al., 2019 Sep, BiofactorsRead this paper
- Paracrine HGF/c-MET enhances the stem cell-like potential and glycolysis of pancreatic cancer cells via activation of YAP/HIF-1alpha. Yan B et al., 2018 Oct 1, Exp Cell ResRead this paper
- Effects of PHA-665752 and vemurafenib combination treatment on in vitro and murine xenograft growth of human colorectal cancer cells with BRAFV600E mutations. Zhi J et al., 2018 Mar, Oncol LettRead this paper
- Biocharacterization of Heat Shock Protein 90 in Acetaminophen-Treated Livers Without Conspicuous Drug Induced Liver Injury. Wu K et al., 2017, Cell Physiol BiochemRead this paper
- Inhibition of peripubertal sheep mammary gland development by cysteamine through reducing progesterone and growth factor production. Zhao Y et al., 2017 Feb, TheriogenologyRead this paper
- Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer. Li H et al., 2015 May, Oncol LettRead this paper
Datasheet
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![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with HGF antibody [N2C1], Internal (GTX111810) diluted at 1:500 and competitor's antibody (sc-7949) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX111810/GTX111810_40093_20170908_WB_competitor_watermark_w_23060500_328.webp)
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