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WB analysis of hypoxia-treated sample using GTX30647 HIF1 alpha antibody. Lane 1 : CoCl2 treated Cos-7 nuclear extract Lane 2 : Untreated Cos-7 nuclear extract Loading : 50microg
WB analysis of hypoxia-treated sample using GTX30647 HIF1 alpha antibody. Lane 1 : CoCl2 treated Cos-7 nuclear extract Lane 2 : Untreated Cos-7 nuclear extract Loading : 50microg
WB analysis of hypoxia-treated sample using GTX30647 HIF1 alpha antibody. Lane 1 : CoCl2 treated Cos-7 nuclear extract Lane 2 : Untreated Cos-7 nuclear extract Loading : 50microg

HIF1 alpha antibody

GTX30647
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityGoat, Hamster, Human, Monkey, Mouse, Primate, Rabbit, Rat
TargetHif1a
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Overview

  • Supplier
    GeneTex
  • Product Name
    HIF1 alpha antibody - Orthogonal Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:100. IHC-P: 5 - 10 microg/ml. IHC-Fr: 5 - 10 microg/ml. FACS: 1:10 - 1:1000. IP: 1:10 - 1:500. ChIP assay: 1:10 - 1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID15251
  • Target name
    Hif1a
  • Target description
    hypoxia inducible factor 1, alpha subunit
  • Target synonyms
    AA959795; ARNT-interacting protein; bHLHe7; bHLHe78; HIF1; HIF-1; HIF1alpha; HIF-1-alpha; HIF1-alpha; hypoxia-inducible factor 1-alpha; MO; MOP1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ61221
  • Protein Name
    Hypoxia-inducible factor 1-alpha
  • Scientific Description
    Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5-[AG]CGTG-3 within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP.
  • Reactivity
    Goat, Hamster, Human, Monkey, Mouse, Primate, Rabbit, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • Endothelial Sirtuin 3 Dictates Glucose Transport to Cardiomyocyte and Sensitizes Pressure Overload-Induced Heart Failure. Zeng H et al., 2020 Jun 2, J Am Heart Assoc
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  • Akt/hypoxia-inducible factor-1alpha signaling deficiency compromises skin wound healing in a type 1 diabetes mouse model. Jing L et al., 2015 Jun, Exp Ther Med
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  • Hypoxia-mediated control of HIF/ARNT machinery in epidermal keratinocytes. Weir L et al., 2011 Jan, Biochim Biophys Acta
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