HIF1 alpha antibody

GeneTex

HIF1 alpha antibody

9

WB: 1:500 - 1:2000. ICC/IF: 1:100. IHC-P: 5 - 10 microg/ml. IHC-Fr: 5 - 10 microg/ml. FACS: 1:10 - 1:1000. IP: 1:10 - 1:500. ChIP assay: 1:10 - 1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

Hif1a

hypoxia inducible factor 1, alpha subunit

HIF-1-alpha, HIF1-alpha, HIF1alpha, MOP1, bHLHe78, hypoxia-inducible factor 1-alpha, ARNT-interacting protein

Rabbit

IgG

Hypoxia-inducible factor 1-alpha

Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5-[AG]CGTG-3 within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP.

Goat, Hamster, Human, Monkey, Mouse, Primate, Rabbit, Rat

2°C to 8°C

12352203