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ProductsBack/HIF1 beta (ARNT) Mouse Monoclonal Antibody [Clone ID: OTI1A1]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ARNT (Cat# RC216724, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARNT(Cat# TA501147). Positive lysates [LY400636] (100ug) and [LC400636] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ARNT (Cat# RC216724, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARNT(Cat# TA501147). Positive lysates [LY400636] (100ug) and [LC400636] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ARNT (Cat# RC216724, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARNT(Cat# TA501147). Positive lysates [LY400636] (100ug) and [LC400636] (20ug) can be purchased separately from OriGene.

HIF1 beta (ARNT) Mouse Monoclonal Antibody [Clone ID: OTI1A1]

CF501147
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetARNT
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    HIF1 beta (ARNT) Mouse Monoclonal Antibody [Clone ID: OTI1A1]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1A1
  • Gene ID405
  • Target name
    ARNT
  • Target description
    aryl hydrocarbon receptor nuclear translocator
  • Target synonyms
    ARNT1, HIF-1-beta, HIF-1beta, HIF1-beta, HIF1B, HIF1BETA, TANGO, bHLHe2, aryl hydrocarbon receptor nuclear translocator, class E basic helix-loop-helix protein 2, dioxin receptor, nuclear translocator, hypoxia inducible factor 1 subunit beta, hypoxia-inducible factor 1, beta subunit
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP27540
  • Protein Name
    Aryl hydrocarbon receptor nuclear translocator
  • Scientific Description
    Carrier-free (BSA/glycerol-free) ARNT mouse monoclonal antibody, clone OTI1A1 (formerly 1A1)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of HIF1 using anti-HIF1 antibody (PB9129). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human HIF1 beta protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF1 antigen affinity purified polyclonal antibody (Catalog # PB9129) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF1 at approximately 49 kDa. The expected band size for HIF1 is at 49 kDa.
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