WB analysis of CoCl?-treated (lane 1) and untreated (lane 2) NIH-3T3 whole cell lysate using GTX85702 HIF3 alpha antibody. Loading : 10 microg Dilution : 1:1600
![Anti-Hif Antibody - Western Blot. Western blot of Affinity Purified anti-Hif antibody shows detection of a band ~72 kD corresponding to mouse Hif3a (arrowhead). Approximately 10 ug of a CoCl2 treated 3T3 cell lysate (lane 1) and control 3T3 cell lysate (lane 2) were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. Treatment of exponentially growing 3T3 cells with 130 uM CoCl2 for 6 h at 37 degree C effectively mimics hypoxia. After blocking the membrane was probed overnight at 4C with the primary antibody diluted to 1:1600. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](https://www.genetex.com/upload/website/prouct_img/normal/GTX85702/HIF3A-antibody_WB_GTX85702-2_w_23061502_912.webp "Anti-Hif Antibody - Western Blot. Western blot of Affinity Purified anti-Hif antibody shows detection of a band ~72 kD corresponding to mouse Hif3a (arrowhead). Approximately 10 ug of a CoCl2 treated 3T3 cell lysate (lane 1) and control 3T3 cell lysate (lane 2) were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. Treatment of exponentially growing 3T3 cells with 130 uM CoCl2 for 6 h at 37 degree C effectively mimics hypoxia. After blocking the membrane was probed overnight at 4C with the primary antibody diluted to 1:1600. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.")
WB analysis of CoCl?-treated (lane 1) and untreated (lane 2) NIH-3T3 whole cell lysate using GTX85702 HIF3 alpha antibody. Loading : 10 microg Dilution : 1:1600
HIF3 alpha antibody
GTX85702
Overview
- SupplierGeneTex
- Product NameHIF3 alpha antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:8000. ELISA: 1:14000-1:80000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- Concentration1.2 mg/ml
- FormulationLiquid
- Gene ID53417
- Target nameHif3a
- Target descriptionhypoxia inducible factor 3, alpha subunit
- Target synonymsbasic-helix-loop-helix-PAS protein MOP7; bHLHe1; bHLHe17; HIF-3-alpha; HIF3-alpha; HIF3-alpha-1; hypoxia inducible factor three alpha; hypoxia-inducible factor 3-alpha; inhibitory PAS domain protein; Ipas; member of PAS protein 7; MO; MOP7; N; neonatal and embryonic PAS protein; NEPAS
- Protein IDQ0VBL6
- Protein NameHypoxia-inducible factor 3-alpha
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- The miRNA-29b Is Downregulated in Placenta During Gestational Diabetes Mellitus and May Alter Placenta Development by Regulating Trophoblast Migration and Invasion Through a HIF3A-Dependent Mechanism. Sun DG et al., 2020, Front Endocrinol (Lausanne)Read more