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ProductsBack/HIF3A / HIF3-Alpha Antibody (aa355-669, clone OTI2D2)

HIF3A / HIF3-Alpha Antibody (aa355-669, clone OTI2D2)

LS-C337024
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetHIF3A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    HIF3A / HIF3-Alpha Antibody (aa355-669, clone OTI2D2)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:150), WB (1:2000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI2D2
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID64344
  • Target name
    HIF3A
  • Target description
    hypoxia inducible factor 3 subunit alpha
  • Target synonyms
    basic-helix-loop-helix-PAS protein MOP7; bHLHe17; class E basic helix-loop-helix protein 17; HIF-3A; HIF3-alpha-1; hypoxia inducible factor 3 alpha subunit; hypoxia-inducible factor 3-alpha; inhibitory PAS domain protein; IPAS; member of PAS protein 7; MOP7; PAS domain-containing protein 7; PASD7
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HIF3A using anti-HIF3A antibody (A03805-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human U2OS whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF3A antigen affinity purified polyclonal antibody (Catalog # A03805-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF3A at approximately 72KD. The expected band size for HIF3A is at 72KD.
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