High Fidelity Core Kit, Kit of thermostable DNA polymerase for high accuracy, dNTPs and reaction buffer

Jena Bioscience

High Fidelity Core Kit, Kit of thermostable DNA polymerase for high accuracy, dNTPs and reaction buffer

5

Other Application

Research Use Only

2.5 unit/ul

High Fidelity Core Kit contains all reagents required for PCR (except template and primer) in one box combining simple handling with high flexibility. The premium quality polymerase, ultrapure dNTPs and the optimized complete reaction buffer ensure superior amplification results. High Fidelity Pol is based on a blend of Taq DNA polymerase and a proofreading enzyme specially designed for highly accurate and efficient amplification. It shows excellent results with extremely long (up to 30 kb), GC-rich or other difficult templates. The enzyme blend includes a highly processive 5 to 3 DNA polymerase and possesses a 5 to 3 polymerization-dependent exonuclease replacement activity. Its inherent 3 to 5 exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase. The enzyme is highly purified and free of bacterial DNA. Fidelity of the enzyme: High Fidelity Pol is characterized by a 4-fold higher fidelity compared to Taq polymerase. ERHigh Fidelity Pol = 3.4 x 10-6 The error rate (ER) of a PCR reaction is calculated using the equation ER = MF/(bp x d), where MF is the mutation frequency, bp is the number of base pairs of the fragment and d is the number of doublings (2d = amount of product / amount of template).

-20°C

41116133