HighFidelity LNA PCR Labeling Kit, Preparation of LNA-labeled DNA probes by PCR

Jena Bioscience

HighFidelity LNA PCR Labeling Kit, Preparation of LNA-labeled DNA probes by PCR

5

Research Use Only

HighFidelity LNA PCR Labeling Kit is designed to produce randomly Locked nucleic acid (LNA)-modified DNA probes by PCR. Such probes are ideally suited for in situ hybridization and Northern Blot experiments. The ribose ring of locked nucleic acids is locked in the ideal conformation for Watson-Crick binding. LNA-modified DNA probes therefore possess an unprecedented thermal stability upon hybridization resulting in an increased sensitivity. LNA-UTP, LNA-CTP, LNA-ATP and LNA-GTP are efficiently incorporated into DNA as substitute for their natural counterpart (dTTP, dCTP, dATP or dGTP, respectively) using an optimized reaction buffer and a High Fidelity Polymerase. 50 % LNA-NTP substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of LNA-NTP/dNTP ratio however, can easily be achieved with the single nucleotide format. The kit contains sufficient reagents for 10 labeling reactions each LNA-NTP (20 microl each (50 % LNA-NTP substitution).

-20°C

41116133