HighYield T7 Biotin16 RNA Labeling Kit, Preparation of randomly Biotin-modified RNA probes by in vitro transcription with Biotin-16-UTP

Jena Bioscience

HighYield T7 Biotin16 RNA Labeling Kit, Preparation of randomly Biotin-modified RNA probes by in vitro transcription with Biotin-16-UTP

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Research Use Only

HighYield T7 Biotin16 RNA Labeling Kit is designed to produce randomly Biotin-modified RNA probes with a 16-atom linker via in vitro transcription. Such probes are ideally suited for in situ hybridization and Northern Blot experiments. The labeling principle is similar to the underlying labeling principles of Biotin RNA Labeling Mix (Roche). Biotin-16-UTP is efficiently incorporated into RNA as substitute for its natural counterpart UTP using an optimized reaction buffer and T7 RNA Labeling Polymerase Mix. 35 % Biotin-16-UTP substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of Biotin-16-UTP/UTP ratio however, can easily be achieved with the single nucleotide format. The resulting Biotin-modified RNA probe with a 16-atom linker can subsequently be detected by HRP- or AP-modified streptavidin. The kit contains sufficient reagents for 30 labeling reactions of 20 microl each (35 % Biotin-16-UTP substitution, 1 mM ATP, GTP, CTP, 0.65 mM UTP, 0.35 mM Biotin-16-UTP).

-20°C

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