HighYield T7 mRNA Synthesis Kit (m5CTP/psi-UTP), Synthesis of 5-methylcytidine and pseudouridine-modified (m)RNA

Jena Bioscience

HighYield T7 mRNA Synthesis Kit (m5CTP/psi-UTP), Synthesis of 5-methylcytidine and pseudouridine-modified (m)RNA

5

Research Use Only

HighYield T7 mRNA Synthesis Kit (m5CTP/psi-UTP) is designed to produce large amounts of 5-methylcytidine and pseudouridine-modified (m)RNA via in vitro transcription with T7 RNA polymerase. The resulting internally modified (m)RNA can subsequently be used for microinjection, transfection or in vitro translation experiments. 5-Methylcytidine and pseudouridine modifications have been shown to increase (m)RNA stability and to reduce immunogenicity. A 5-cap structure further increases translation efficiency. 5-capping can be achieved by a) co-transcriptional capping with cap analoga (e.g. ARCA) or b) post-transcriptional capping with capping enzymes. The kit contains sufficient reagents for 15 labeling reactions a 20 microl (7.5 mM GTP, 7.5 mM 5-Methyl-CTP, 7.5 mM Pseudo-UTP, 7.5 mM ATP). An individual optimization of 5-methyl-CTP and Pseudo-UTP concentration as well cap analoga incorporation can easily be achieved with the single nucleotide format. A 20 microl reaction yields about 100-130 microg RNA after 30 min incubation (1 microg T7 control template, 1.4 kb RNA transcript). Yields may however vary depending on the template (promotor design, sequence length, secondary structure formation)).

-20°C

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