HighYield T73M Aptamer Synthesis Kit (2F-dATP), Synthesis of 2-Fluoro-modified RNA

Jena Bioscience

HighYield T73M Aptamer Synthesis Kit (2F-dATP), Synthesis of 2-Fluoro-modified RNA

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Research Use Only

HighYield T73M Aptamer Synthesis Kit (2F-dATP) is designed to produce large amounts of 2-Fluoro-modified RNA via in vitro transcription with T73M RNA polymerase. 2-fluoro modified RNA exhibits an increased RNA stability and is ideally suited for aptamer preparation. A modified T7 RNA polymerase (T73M RNA polymerase) ensures the efficient incorporation of 2-fluoro modified dATP instead of is natural counterpart ATP. The kit contains sufficient reagents for 30 reactions a 20 microl (7.5 mM GTP, 7.5 mM 2-Fluoro-dATP, 7.5 mM UTP, 7.5 mM CTP). An individual optimization of 2-Fluoro-dATP concentration can easily be achieved with the single nucleotide format. A 20 microl reaction yields about 20 microg RNA after 30 min incubation (1 microg T7 control template, 1.4 kb RNA transcript). Yields may however vary depending on the template (promotor design, sequence length, secondary structure formation).

-20°C

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