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ProductsBack/HIP antibody [N3C3]

HIP antibody [N3C3]

GTX120043
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetST13
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Overview

  • Supplier
    GeneTex
  • Product Name
    HIP antibody [N3C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:5000-1:20000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6767
  • Target name
    ST13
  • Target description
    ST13 Hsp70 interacting protein
  • Target synonyms
    AAG2; aging-associated protein 2; FAM10A1; FAM10A4; heat shock 70kD protein binding protein; HIP; HOP; hsc70-interacting protein; Hsp70-interacting protein; HSPABP; HSPABP1; P48; PRO0786; progesterone receptor-associated p48 protein; putative tumor suppressor ST13; renal carcinoma antigen NY-REN-33; SNC6; suppression of tumorigenicity 13 (colon carcinoma) (Hsp70 interacting protein); suppression of tumorigenicity 13 protein; testis secretory sperm-binding protein Li 233m
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP50502
  • Protein Name
    Hsc70-interacting protein
  • Scientific Description
    The protein encoded by this gene is an adaptor protein that mediates the association of the heat shock proteins HSP70 and HSP90. This protein has been shown to be involved in the assembly process of glucocorticoid receptor, which requires the assistance of multiple molecular chaperones. The expression of this gene is reported to be downregulated in colorectal carcinoma tissue suggesting that is a candidate tumor suppressor gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ST13 using anti-ST13 antibody (A05692-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human SK-OV-3 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ST13 antigen affinity purified polyclonal antibody (Catalog # A05692-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ST13 at approximately 45-54 kDa. The expected band size for ST13 is at 41 kDa.
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