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HIP12 Polyclonal Antibody

BS-9067R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetHIP1R
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    HIP12 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IF(IHC-P)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID9026
  • Target name
    HIP1R
  • Target description
    huntingtin interacting protein 1 related
  • Target synonyms
    HIP12; HIP-12; HIP1-related protein; HIP3; huntingtin interacting protein 12; huntingtin-interacting protein 1-related protein; ILWEQ
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: Rat brain tissue All lanes: HIP1R antibody at 2.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 120, 71 kDa Observed band size: 120 kDa
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Figure 1. Western blot analysis of HIP1R using anti-HIP1R antibody (A05059-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human T-47D whole cell lysates, Lane 5: human Raji whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: rat heart tissue lysates, Lane 8: rat pancreas tissue ltysates, Lane 9: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIP1R antigen affinity purified polyclonal antibody (Catalog # A05059-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIP1R at approximately 120 kDa. The expected band size for HIP1R is at 120 kDa.
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Immunohistochemical staining of human cerebral cortex, colon, kidney and testis using Anti-HIP1R antibody HPA038135 (A) shows similar protein distribution across tissues to independent antibody HPA038136 (B).
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