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Formalin-fixed and paraffin embedded rat brain labeled with Anti-HIP2 Polyclonal Antibody, Unconjugated (bs-8379R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-HIP2 Polyclonal Antibody, Unconjugated (bs-8379R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-HIP2 Polyclonal Antibody, Unconjugated (bs-8379R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

HIP2 Polyclonal Antibody

BS-8379R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetUBE2K
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    HIP2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3093
  • Target name
    UBE2K
  • Target description
    ubiquitin conjugating enzyme E2 K
  • Target synonyms
    E2 ubiquitin-conjugating enzyme K; E2(25K); E2-25K; HIP2; HIP-2; huntingtin-interacting protein 2; HYPG; LIG; UBC1; ubiquitin carrier protein; ubiquitin conjugating enzyme E2K; ubiquitin-conjugating enzyme E2 K; ubiquitin-conjugating enzyme E2(25K); ubiquitin-conjugating enzyme E2-25 KDA; ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast); ubiquitin-protein ligase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP61086
  • Protein Name
    Ubiquitin-conjugating enzyme E2 K
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HIP2/LIG/UBE2K using anti-HIP2/LIG/UBE2K antibody (A05895-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIP2/LIG/UBE2K antigen affinity purified polyclonal antibody (Catalog # A05895-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIP2/LIG/UBE2K at approximately 22 kDa. The expected band size for HIP2/LIG/UBE2K is at 22 kDa.
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