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ProductsBack/HIST1H3A (Ab-122) Antibody
Western Blot Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, Rat brain tissue, Rat liver tissue, Rat kidney tissue, Mouse liver tissue, Mouse kidney tissue All lanes: HIST1H3A antibody at 1.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 16 kDa Observed band size: 16 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, Rat brain tissue, Rat liver tissue, Rat kidney tissue, Mouse liver tissue, Mouse kidney tissue All lanes: HIST1H3A antibody at 1.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 16 kDa Observed band size: 16 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, Rat brain tissue, Rat liver tissue, Rat kidney tissue, Mouse liver tissue, Mouse kidney tissue All lanes: HIST1H3A antibody at 1.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 16 kDa Observed band size: 16 kDa

HIST1H3A (Ab-122) Antibody

CSB-PA010418OA122NHIBHU
Cusabio
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetH3C1
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Overview

  • Supplier
    Cusabio
  • Product Name
    HIST1H3A (Ab-122) Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8350
  • Target name
    H3C1
  • Target description
    H3 clustered histone 1
  • Target synonyms
    H3/A, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A, histone H3.1, H3 histone family, member A, histone 1, H3a, histone H3/a, histone cluster 1 H3 family member a, histone cluster 1, H3a
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP68431
  • Protein Name
    Histone H3.1
  • Scientific Description
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Histone H3 using anti-Histone H3 antibody (A12477-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human 22RV1 whole cell lysates, Lane 3: human CACO-2 whole cell lysates, Lane 4: human CCRF-CEM whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human HepG2 whole cell lysates, Lane 7: human THP-1 whole cell lysates, Lane 8: human U2OS whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Histone H3 antigen affinity purified polyclonal antibody (Catalog # A12477-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Histone H3 at approximately 17 kDa. The expected band size for Histone H3 is at 15 kDa.
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Western blot All lanes: HIST1H3A antibody at 1.83ug/ml Lane 1: Mouse liver tissue Lane 2: Mouse lung tissue Lane 3: MCF-7 whole cell lysate Lane 4: Hela whole cell lysate Lane 5: Jurkat whole cell lysate Lane 6: NIH/3T3 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 15 kDa Observed band size: 15 kDa
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