Western blot using GeneTexs affinity purified anti-histone H2AvD pS137 antibody shows detection of a band at ~15 kDa corresponding to phosphorylated H2AvD (lane 2 arrow-head). Lanes contain either mock-irradiated (lane 1) or 4000-RAD gamma irradiated (lane 2) Drosophila melanogaster (3rd instar) larvae brain WC lysate separated on by SDS-PAGE and transferred to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:500. Washes and reaction with secondary antibody followed incubation. Use HRP conjugated Gt-a-Rabbit IgG [H&L] MX and ECL for detection.
Western blot using GeneTexs affinity purified anti-histone H2AvD pS137 antibody shows detection of a band at ~15 kDa corresponding to phosphorylated H2AvD (lane 2 arrow-head). Lanes contain either mock-irradiated (lane 1) or 4000-RAD gamma irradiated (lane 2) Drosophila melanogaster (3rd instar) larvae brain WC lysate separated on by SDS-PAGE and transferred to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:500. Washes and reaction with secondary antibody followed incubation. Use HRP conjugated Gt-a-Rabbit IgG [H&L] MX and ECL for detection.
Histone H2A.vS137ph (phospho Ser137) antibody
GTX48733
ApplicationsElectron Microscopy, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
ReactivityDrosophila
TargetHis2Av
Overview
- SupplierGeneTex
- Product NameHistone H2A.vS137ph (phospho Ser137) antibody
- Delivery Days Customer9
- Antibody SpecificityReactivity occurs against Drosophila H2AvD pS137 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated Drosophila H2AvD is minimal by ELISA.
- Application Supplier NoteWB: 1:400-1:1600. IHC-P: 2 microg/mL. ELISA: 1:29000-1:30000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsElectron Microscopy, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.1 mg/ml
- ConjugateUnconjugated
- Gene ID43229
- Target nameHis2Av
- Target descriptionHistone H2A variant
- Target synonyms*i H2av; 5499; CG5499; CG5499-PA; CG5499-PB; dHis2Av; Dmel\CG5499; Dmel_CG5499; gammaH2AV; gamma-H2AV; gamma-H2AvD; gammaH2Ax; gamma-HIS2AV; H2A; H2A.F/Z; H2A.V; H2A.X; H2A.Z; H2AV; H2AV_DROM; H2avD; H2AX; H2AZ; HIS; His2; His2A; His2AV; HIS2AVD; His2Av-PA; His2Av-PB; HisH2Av; Hist; Hist2av; histone; histone 2A; histone H2A; histone H2A.v; histone H2Av; histone2A; Histone-2A; histone2Av; Histone-2av; histone2AvD; l(3)05146; l(3)810; l(3)97Dd; l(3)L1602
- HostRabbit
- IsotypeIgG
- Protein IDP08985
- Protein NameHistone H2A.v
- Scientific DescriptionVariant histones H2A are synthesized throughout the cell cycle and are very different from classical S-phase regulated H2A. H2AvD is vital for viability, but the exact function of variant histones H2A is not known. H2A is a core component of the nucleosome, an octamer containing two molecules each of H2A, H2B, H3 and H4. The octamer wraps approximately 146 bp of DNA. HsAvD is expressed both maternally and zygotically and is found in embryos through to adults (female only). The human homologue, H2AX, is phosphorylated by ATM protein kinase when double strand DNA breaks occur. In mouse, H2AX knock out mice have an increased incidence of cancer.
- ReactivityDrosophila
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Metabolically active and polyploid renal tissues rely on graded cytoprotection to drive developmental and homeostatic stress resilience. Burbridge K et al., 2021 Apr 15, DevelopmentRead more