![Histone H2A.XS139ph (phospho Ser139) antibody [HL1299] detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Histone H2A.XS139ph (phospho Ser139) stained by Histone H2A.XS139ph (phospho Ser139) antibody [HL1299] (GTX636713) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Histone H2A.XS139ph (phospho Ser139) antibody [HL1299] detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Histone H2A.XS139ph (phospho Ser139) stained by Histone H2A.XS139ph (phospho Ser139) antibody [HL1299] (GTX636713) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX636713/GTX636713_44620_20220408_IHC-P_M_22071401_185.webp)
Histone H2A.XS139ph (phospho Ser139) antibody [HL1299] detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Histone H2A.XS139ph (phospho Ser139) stained by Histone H2A.XS139ph (phospho Ser139) antibody [HL1299] (GTX636713) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Histone H2A.XS139ph (phospho Ser139) antibody [HL1299]
GTX636713
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetH2AX
Overview
- SupplierGeneTex
- Product NameHistone H2A.XS139ph (phospho Ser139) antibody [HL1299]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1299
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3014
- Target nameH2AX
- Target descriptionH2A.X variant histone
- Target synonymsH2A.X, H2A/X, H2AFX, histone H2AX, H2A histone family member X, H2AX histone, histone H2A.x
- HostRabbit
- IsotypeIgG
- Protein IDP16104
- Protein NameHistone H2AX
- Scientific DescriptionHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![Histone H2A.XS139ph (phospho Ser139) antibody [HL1299] detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H2A.XS139ph (phospho Ser139) stained by Histone H2A.XS139ph (phospho Ser139) antibody [HL1299] (GTX636713) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm. Histone H2A.XS139ph (phospho Ser139) antibody [HL1299] detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H2A.XS139ph (phospho Ser139) stained by Histone H2A.XS139ph (phospho Ser139) antibody [HL1299] (GTX636713) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636713/GTX636713_44620_20220429_ICC_IF_treatment_Cisplatin_w_23061202_303.webp)
![Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody [HL1299] (GTX636713) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody [HL1299] (GTX636713) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636713/GTX636713_45271_20231229_WB_treatment_Cisplatin_24090423_890.webp)





![Histone H2A.X antibody [N1N2], N-term detects Histone H2A.X protein at nucleus by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H2A.X protein stained by Histone H2A.X antibody [N1N2], N-term (GTX108297) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX108297/GTX108297_39750_20141205_IFA_w_23060120_599.webp)
![WB analysis of A375, HEK293, HeLa and SK-MEL-2 whole cell lysates using GTX60914 Histone H2A.X antibody [RM214]. Dilution : 0.5μg/ml](https://www.genetex.com/upload/website/prouct_img/normal/GTX60914/GTX60914_20200909_WB_253_w_23061123_238.webp)
![Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] (GTX628789) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX628789/GTX628789_41190_20221216_WB_treatment_cisplatin_22122018_295.webp)
![Histone H2A.XS139ph (phospho Ser139) antibody [GT1021] detects Histone H2A.XS139ph (phospho Ser139) protein at Nucleus by immunofluorescent analysis. Sample: 10uM Cisplatin for 24 hrs HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: Histone H2A.XS139ph (phospho Ser139) protein stained by Histone H2A.XS139ph (phospho Ser139) antibody [GT1021] (GTX628996) diluted at 1:500. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX628996/GTX628996_41288_IFA_w_23061202_265.webp)
![ICC/IF analysis of cells using GTX80694 Histone H2A.XS139ph (phospho Ser139) antibody [3F2].](https://www.genetex.com/upload/website/prouct_img/normal/GTX80694/GTX80694_2034_ICC-IF_w_23061322_855.webp)