More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Histone H3 antibody [RM188]
ICC/IF analysis of HeLa cells using GTX60888 Histone H3 antibody [RM188]. Red : Primary antibody Green : Actin
ICC/IF analysis of HeLa cells using GTX60888 Histone H3 antibody [RM188]. Red : Primary antibody Green : Actin
ICC/IF analysis of HeLa cells using GTX60888 Histone H3 antibody [RM188]. Red : Primary antibody Green : Actin

Histone H3 antibody [RM188]

GTX60888
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetH3C1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    Histone H3 antibody [RM188]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.01 microg/mL - 0.25 microg/mL. ICC/IF: 1 microg/mL - 5 microg/mL. ELISA: 0.2 microg/mL - 1 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RM188
  • Conjugate
    Unconjugated
  • Gene ID8350
  • Target name
    H3C1
  • Target description
    H3 clustered histone 1
  • Target synonyms
    H3/A, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A, histone H3.1, H3 histone family, member A, histone 1, H3a, histone H3/a, histone cluster 1 H3 family member a, histone cluster 1, H3a
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Anti-H3K18ac Polyclonal Antibody
Product group Antibodies
BPS Bioscience
Anti-H3K18ac Polyclonal Antibody25236
ApplicationsDot Blot, Western Blot, ELISA
ReactivityHuman
TargetH3C1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-MonoMethyl-Histone H3-R17 Antibody144-03151
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat, Other Species
TargetH3C1
  • SizePrice
Figure 1. Western blot analysis of Histone H3 using anti-Histone H3 antibody (A12477-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human 22RV1 whole cell lysates, Lane 3: human CACO-2 whole cell lysates, Lane 4: human CCRF-CEM whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human HepG2 whole cell lysates, Lane 7: human THP-1 whole cell lysates, Lane 8: human U2OS whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Histone H3 antigen affinity purified polyclonal antibody (Catalog # A12477-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Histone H3 at approximately 17 kDa. The expected band size for Histone H3 is at 15 kDa.
Product group Antibodies
Boster Bio
Anti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband(r)A12477-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetH3C1
  • SizePrice
Product group Antibodies
Abbexa
HIST1H3A Antibody (HRP)ABX313465
ApplicationsELISA
  • SizePrice
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous tubules.
Product group Antibodies
Atlas Antibodies
Anti-HIST1H3A AntibodyAMAB91331
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetH3C1
  • SizePrice
Product group Antibodies
Biosynth
Histone H3 antibody20-HS68
  • SizePrice
Product group Antibodies
Biomatik
Acetyl-HIST1H3A (K115) Polyclonal AntibodyCAC12821
ApplicationsELISA, ImmunoCytoChemistry
TargetH3C1
  • SizePrice
TT lysates probed with Histone H3.1 Polyclonal Antibody, Unconjugated (bs-17422R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Product group Antibodies
References
Bioss
Histone H3.1 Polyclonal AntibodyBS-17422R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Equine, Human, Mouse, Porcine, Primate, Rabbit, Rat, Sheep
TargetH3C1
  • SizePrice
Western blot All lanes: HIST1H3A antibody at 1.83ug/ml Lane 1: Mouse liver tissue Lane 2: Mouse lung tissue Lane 3: MCF-7 whole cell lysate Lane 4: Hela whole cell lysate Lane 5: Jurkat whole cell lysate Lane 6: NIH/3T3 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 15 kDa Observed band size: 15 kDa
Product group Antibodies
Cusabio
HIST1H3A AntibodyCSB-PA010418ESR1HU
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetH3C1
  • SizePrice
Product group Antibodies
Assay Genie
MonoMethyl-Histone H3-K27 AntibodyCAB2361
ReactivityHuman
TargetH3C1
  • SizePrice