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ProductsBack/Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade
ChIP analysis of sheared chromatin from 10? HeLa cells treated with colcemid using GTX60329 Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade. A titration of the antibody consisting of 1, 5 and 10 microl per ChIP experiment was analysed. Additionally, ChIP was performed after incubation of the antibody with 5 nmol blocking peptide for 1 hour at room temperature. IgG (5 microg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the active genes c-fos and RPL30 and for the inactive gene MYOD. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ChIP analysis of sheared chromatin from 10? HeLa cells treated with colcemid using GTX60329 Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade. A titration of the antibody consisting of 1, 5 and 10 microl per ChIP experiment was analysed. Additionally, ChIP was performed after incubation of the antibody with 5 nmol blocking peptide for 1 hour at room temperature. IgG (5 microg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the active genes c-fos and RPL30 and for the inactive gene MYOD. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ChIP analysis of sheared chromatin from 10? HeLa cells treated with colcemid using GTX60329 Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade. A titration of the antibody consisting of 1, 5 and 10 microl per ChIP experiment was analysed. Additionally, ChIP was performed after incubation of the antibody with 5 nmol blocking peptide for 1 hour at room temperature. IgG (5 microg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the active genes c-fos and RPL30 and for the inactive gene MYOD. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade

GTX60329
GeneTex
ApplicationsDot Blot, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
Product group Antibodies
TargetH3C1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:250 - 1:500. ICC/IF: 1:200. IP: 5 microl. Dot: 1:20,000. ELISA: 1:100 - 1:500. ChIP assay: 1 microl. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Dot Blot, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8350
  • Target name
    H3C1
  • Target description
    H3 clustered histone 1
  • Target synonyms
    H3/A, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A, histone H3.1, H3 histone family, member A, histone 1, H3a, histone H3/a, histone cluster 1 H3 family member a, histone cluster 1, H3a
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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