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ChIP analysis of sheared chromatin from 1.5x10? U2OS cells using GTX60328 Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody. A titration of the antibody consisting of 5 and 15 microl per ChIP experiment was analysed. IgG (5 microg/IP) was used as negative IP control. QPCR was performed with primers for the ALDOA (fructose-bisphosphate aldolase A) promoter and for the coding region of the myogenic differentiation gene (MYOD), a gene that is inactive at normal conditions. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ChIP analysis of sheared chromatin from 1.5x10? U2OS cells using GTX60328 Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody. A titration of the antibody consisting of 5 and 15 microl per ChIP experiment was analysed. IgG (5 microg/IP) was used as negative IP control. QPCR was performed with primers for the ALDOA (fructose-bisphosphate aldolase A) promoter and for the coding region of the myogenic differentiation gene (MYOD), a gene that is inactive at normal conditions. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ChIP analysis of sheared chromatin from 1.5x10? U2OS cells using GTX60328 Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody. A titration of the antibody consisting of 5 and 15 microl per ChIP experiment was analysed. IgG (5 microg/IP) was used as negative IP control. QPCR was performed with primers for the ALDOA (fructose-bisphosphate aldolase A) promoter and for the coding region of the myogenic differentiation gene (MYOD), a gene that is inactive at normal conditions. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody

GTX60328
GeneTex
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
Product group Antibodies
TargetH3C1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:250. ICC/IF: 1:500. Dot: 1:20,000. ELISA: 1:1,000 - 1:4,000. ChIP assay: 15 microl. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Dot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8350
  • Target name
    H3C1
  • Target description
    H3 clustered histone 1
  • Target synonyms
    H3/A, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A, histone H3.1, H3 histone family, member A, histone 1, H3a, histone H3/a, histone cluster 1 H3 family member a, histone cluster 1, H3a
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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