
ChIP analysis of HeLa cells using GTX60825 Histone H3R17me2K18ac (Asymmetric Di-methyl Arg17/Acetyl Lys18) antibody - ChIP grade. The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete human X-chromosome and a zoomin to a 500 kb region (figure 2A and B), and in two regions on chromosome 14 and 3 surrounding the c-fos and EIF4A2 positive control genes (figure 2C and D, respectively). Antibody amount : 1μg
Histone H3R17me2K18ac (Asymmetric Di-methyl Arg17/Acetyl Lys18) antibody - ChIP grade
GTX60825
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetH3C1
Overview
- SupplierGeneTex
- Product NameHistone H3R17me2K18ac (Asymmetric Di-methyl Arg17/Acetyl Lys18) antibody - ChIP grade
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1,000. ICC/IF: 1:500. Dot: 1:20,000. ELISA: 1:1,000. ChIP assay: 1-5 microg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.94 mg/ml
- ConjugateUnconjugated
- Gene ID8350
- Target nameH3C1
- Target descriptionH3 clustered histone 1
- Target synonymsH3/A, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A, histone H3.1, H3 histone family, member A, histone 1, H3a, histone H3/a, histone cluster 1 H3 family member a, histone cluster 1, H3a
- HostRabbit
- IsotypeIgG
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203











