More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody
Histone3 (di-methyl Arg2) antibody detects HIST1H3A protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 15 % SDS-PAGE Histone3 (di-methyl Arg2) antibody (GTX124114) dilution: 1:1000
Histone3 (di-methyl Arg2) antibody detects HIST1H3A protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 15 % SDS-PAGE Histone3 (di-methyl Arg2) antibody (GTX124114) dilution: 1:1000
Histone3 (di-methyl Arg2) antibody detects HIST1H3A protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 15 % SDS-PAGE Histone3 (di-methyl Arg2) antibody (GTX124114) dilution: 1:1000

Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody

GTX124114
GeneTex
ApplicationsDot Blot, Western Blot
Product group Antibodies
ReactivityHuman
TargetH3C1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Dot Blot, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.72 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8350
  • Target name
    H3C1
  • Target description
    H3 clustered histone 1
  • Target synonyms
    H3/A, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A, histone H3.1, H3 histone family, member A, histone 1, H3a, histone H3/a, histone cluster 1 H3 family member a, histone cluster 1, H3a
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Anti-H3K18ac Polyclonal Antibody
Product group Antibodies
BPS Bioscience
Anti-H3K18ac Polyclonal Antibody25236
ApplicationsDot Blot, Western Blot, ELISA
ReactivityHuman
TargetH3C1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-MonoMethyl-Histone H3-R17 Antibody144-03151
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat, Other Species
TargetH3C1
  • SizePrice
Product group Antibodies
Abbexa
HIST1H3A Antibody (HRP)ABX313465
ApplicationsELISA
  • SizePrice
Product group Antibodies
Biosynth
Histone H3 antibody20-HS68
  • SizePrice
Figure 1. Western blot analysis of Histone H3 using anti-Histone H3 antibody (A12477-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human 22RV1 whole cell lysates, Lane 3: human CACO-2 whole cell lysates, Lane 4: human CCRF-CEM whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human HepG2 whole cell lysates, Lane 7: human THP-1 whole cell lysates, Lane 8: human U2OS whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Histone H3 antigen affinity purified polyclonal antibody (Catalog # A12477-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Histone H3 at approximately 17 kDa. The expected band size for Histone H3 is at 15 kDa.
Product group Antibodies
Boster Bio
Anti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband(r)A12477-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetH3C1
  • SizePrice
Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody (GTX127985) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
Histone H3 antibodyGTX127985
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityDrosophila, Human
TargetH3C1
  • SizePrice
Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody (GTX129546) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
References
GeneTex
Histone H3 antibodyGTX129546
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityDrosophila, Human, Mouse, Rat
TargetH3C1
  • SizePrice
Histone H3 (tri-Methyl Lys27) antibody detects Histone H3 (tri-Methyl Lys27) protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (tri-Methyl Lys27) antibody (GTX129774) diluted by 1:1000.
Product group Antibodies
GeneTex
Histone H3K27me3 (Tri-methyl Lys27) antibodyGTX129774
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetH3C1
  • SizePrice
Histone H3 (di-Methyl Lys79) antibody detects Histone H3 (di-Methyl Lys79) protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (di-Methyl Lys79) antibody (GTX129776) diluted by 1:1000.
Product group Antibodies
GeneTex
Histone H3K79me2 (Di-methyl Lys79) antibodyGTX129776
ApplicationsWestern Blot
ReactivityHuman
TargetH3C1
  • SizePrice
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3T3ph (phospho Thr3) antibody (GTX130458) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Product group Antibodies
GeneTex
Histone H3T3ph (phospho Thr3) antibodyGTX130458
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetH3C1
  • SizePrice