ICC/IF analysis of C6 cells treated with/without TSA (1 microM at 37oC for 18 hours) using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138]. Orange: Primary antibody Blue: DAPI
![ICC/IF analysis of NIH3T3 cells treated with/without TSA (1 microM at 37oC for 18 hours) using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138]. Orange: Primary antibody Blue: DAPI](https://www.genetex.com/upload/website/prouct_img/normal/GTX00775/GTX00775_20191101_AP_002_28_w_23053121_342.webp "ICC/IF analysis of NIH3T3 cells treated with/without TSA (1 microM at 37oC for 18 hours) using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138]. Orange: Primary antibody Blue: DAPI")
![ICC/IF analysis of U2OS cells treated with/without TSA (1 microM at 37oC for 18 hours) using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138]. Orange: Primary antibody Blue: DAPI](https://www.genetex.com/upload/website/prouct_img/normal/GTX00775/GTX00775_20191101_AP_002_29_w_23053121_294.webp "ICC/IF analysis of U2OS cells treated with/without TSA (1 microM at 37oC for 18 hours) using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138]. Orange: Primary antibody Blue: DAPI")
![IHC-P analysis of rat brain tissue section using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138]. Dlution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX00775/GTX00775_20191101_AP_003_24_w_23053121_944.webp "IHC-P analysis of rat brain tissue section using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138]. Dlution : 1:100")
![IHC-P analysis of hmuan appendix tissue section using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138]. Dlution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX00775/GTX00775_20191101_AP_003_25_w_23053121_619.webp "IHC-P analysis of hmuan appendix tissue section using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138]. Dlution : 1:100")
![IHC-P analysis of mouse kidney tissue section using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138]. Dlution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX00775/GTX00775_20191101_AP_003_26_w_23053121_985.webp "IHC-P analysis of mouse kidney tissue section using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138]. Dlution : 1:100")
![WB analysis of HeLa and C6 whole cell lysates using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138]. Dilution : 1:1000 Loading : 25microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX00775/GTX00775_20200327_WB_1_w_23053121_278.webp "WB analysis of HeLa and C6 whole cell lysates using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138]. Dilution : 1:1000 Loading : 25microg")
![Untreated (-) and treated (+) HeLa whole cell extracts (30 microg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4K5ac (acetyl Lys5) antibody [GT1138] (GTX00775) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX00775/GTX00775_40000000002_20200306_WB_treatment_Trichostatin_A_w_23053121_239.webp "Untreated (-) and treated (+) HeLa whole cell extracts (30 microg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4K5ac (acetyl Lys5) antibody [GT1138] (GTX00775) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
ICC/IF analysis of C6 cells treated with/without TSA (1 microM at 37oC for 18 hours) using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138]. Orange: Primary antibody Blue: DAPI
Histone H4K5ac (acetyl Lys5) antibody [GT1138]
GTX00775
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetH4-16
Overview
- SupplierGeneTex
- Product NameHistone H4K5ac (acetyl Lys5) antibody [GT1138] - Recombinant antibody, Orthogonal Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1138
- ConjugateUnconjugated
- Gene ID121504
- Target nameH4-16
- Target descriptionH4 histone 16
- Target synonymsH4/p; H4C1; H4C11; H4C12; H4C13; H4C14; H4C15; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; HIST4H4; histone 4, H4; histone cluster 4 H4; histone H4
- HostRabbit
- IsotypeIgG
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203