
Staining of peripheral blood lymphocytes using HLA Class I antibody (GTX27855).
HLA Class I antibody [W6/32]
GTX27855
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityHuman
Overview
- SupplierGeneTex
- Product NameHLA Class I antibody [W6/32]
- Delivery Days Customer9
- Application Supplier NoteFor IHC(frozen) a three-step avidin-biotin complex system, a dilution of 1:50 to 1:100 may be used as a guideline. In general, the 0.05M Borate pH 8.0 containing 0.15M Sodium Chloride, 0.05% Sodium Azide, is a good dilutent to use with most antibodies. Optimal dilutions/concentrations should be determined by the researcher.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDW6/32
- ConjugateUnconjugated
- HostMouse
- IsotypeIgG2a
- Scientific DescriptionHLA-class I antigens are widely distributed on human nucleated cells. The intensity of HLA-ABC maybe altered in pathological states e.g. malignant cells may loose HLA-ABC, whereas hepatocytes in alcoholic hepatitis, biliary cirrhosis and chronic active hepatitis may show enhanced expression of HLA-ABC.
- ReactivityHuman
- Storage Instruction2°C to 8°C
- UNSPSC12352203
References
- Sekijima M, Sahara H, Shimizu A, et al. Preparation of hybrid porcine thymus containing non-human primate thymic epithelial cells in miniature swine. Xenotransplantation. 2019,26(6):e12543. doi: 10.1111/xen.12543Read this paper


![HLA-A antibody [N1C2] immunoprecipitates HLA-A protein in IP experiments. IP samples: A549 whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of HLA-A protein by 4 μg HLA-A antibody [N1C2] (GTX114080) 10 % SDS-PAGE The immunoprecipitated HLA-A protein was detected by HLA-A antibody [N1C2] (GTX114080) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]](https://www.genetex.com/upload/website/prouct_img/normal/GTX114080/GTX114080_40611_IP_w_23060501_714.webp)
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HLA class I antibody [HL1882] (GTX637631) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637631/GTX637631_T-44837_20240729_WB_multiple_B_24080622_850.webp)
![FACS analysis of human peripheral blood lymphocytes using GTX75803 HLA-A2 antibody [BB7.2].](https://www.genetex.com/upload/website/prouct_img/normal/GTX75803/GTX75803_3788_FACS_w_23061322_791.webp)
![FACS analysis of human peripheral blood monocytes using GTX75804 HLA-A2 antibody [BB7.2] (FITC).](https://www.genetex.com/upload/website/prouct_img/normal/GTX75804/GTX75804_3802_FACS_w_23061322_200.webp)
![FACS analysis of human peripheral blood lymphocytes using GTX75805 HLA-A2 antibody [BB7.2] (PE).](https://www.genetex.com/upload/website/prouct_img/normal/GTX75805/GTX75805_3805_FACS_w_23061322_233.webp)