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HLA-DRA Antibody (Biotin)

ABX105535
Abbexa
ApplicationsELISA
DownloadDatasheet
Product group Antibodies
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Overview

  • Supplier
    Abbexa
  • Product Name
    HLA-DRA Antibody (Biotin)
  • Delivery Days Customer
    12
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Biotin
  • Host
    Rabbit
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HLA-DRA using anti-HLA-DRA antibody (A01195). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human Ramos whole cell lysates, Lane 4: human HMy2.CIR whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLA-DRA antigen affinity purified polyclonal antibody (Catalog # A01195) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HLA-DRA at approximately 37 kDa. The expected band size for HLA-DRA is at 29 kDa.
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