Analysis of cytolytical activity of human polyclonal NK cells on target melanoma cells. Blocking of HLA-G1 on transfectants with anti-human HLA-G (87G) restored specific lysis. Target cells: M8 cell line transfected with empty vector (column 1) and with HLA-G1 cDNA (columns 2-6).Blocking antibodies: Column 1-2: noneColumn 3: Isotype mouse IgG2a controlColumn 4: anti-human HLA-G (87G) purifiedColumn 5: anti-human HLA-G (87G) F(ab)2 fragmentColumn 6: anti-human HLA-G (MEM-G/9)
Analysis of cytolytical activity of human polyclonal NK cells on target melanoma cells. Blocking of HLA-G1 on transfectants with anti-human HLA-G (87G) restored specific lysis. Target cells: M8 cell line transfected with empty vector (column 1) and with HLA-G1 cDNA (columns 2-6).Blocking antibodies: Column 1-2: noneColumn 3: Isotype mouse IgG2a controlColumn 4: anti-human HLA-G (87G) purifiedColumn 5: anti-human HLA-G (87G) F(ab)2 fragmentColumn 6: anti-human HLA-G (MEM-G/9)
HLA-G antibody [87G] (Biotin)
GTX80253
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityHuman
TargetHLA-G
Overview
- SupplierGeneTex
- Product NameHLA-G antibody [87G] (Biotin)
- Delivery Days Customer9
- Antibody SpecificityThis antibody recognizes both membrane-bound and soluble forms of HLA-G (HLA-G1 and HLA-G5). The antibody 87G blocks interaction of HLA-G with inhibitory receptors.,We do not recommend use of this product for Mouse,Rat.
- ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID87G
- Concentration1 mg/ml
- ConjugateBiotin
- Gene ID3135
- Target nameHLA-G
- Target descriptionmajor histocompatibility complex, class I, G
- Target synonymsb2 microglobulin; HLA class I histocompatibility antigen, alpha chain G; HLA G antigen; HLA-G histocompatibility antigen, class I, G; MHC class I antigen G; MHC class Ib antigen; MHC-G; mutant MHC class I antigen; mutant MHC class Ib antigen
- HostMouse
- IsotypeIgG2a
- Scientific DescriptionHLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]
- ReactivityHuman
- Storage Instruction2°C to 8°C
- UNSPSC12352203
References
- Umbilical cord blood-derived mesenchymal stromal cells promote myeloid-derived suppressor cell proliferation by secreting HLA-G to reduce acute graft-versus-host disease after hematopoietic stem cell transplantation. Yang S et al., 2020 Dec, CytotherapyRead more