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ProductsBack/HLAC (HLA-C) Rabbit Polyclonal Antibody
WB Suggested Anti-HLA-C Antibody Titration: 1.0 ug/ml Positive Control: MDA-MB-435S Whole Cell
WB Suggested Anti-HLA-C Antibody Titration: 1.0 ug/ml Positive Control: MDA-MB-435S Whole Cell
WB Suggested Anti-HLA-C Antibody Titration: 1.0 ug/ml Positive Control: MDA-MB-435S Whole Cell

HLAC (HLA-C) Rabbit Polyclonal Antibody

TA358332
OriGene
ApplicationsWestern Blot
Product group Antibodies
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    HLAC (HLA-C) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Host
    Rabbit
  • Protein IDQ29960
  • Protein Name
    HLA class I histocompatibility antigen, Cw-16 alpha chain
  • Scientific Description
    HLA-C Antibody - C-terminal region
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of HLA-C using anti-HLA-C antibody (PB9377). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLA-C antigen affinity purified polyclonal antibody (Catalog # PB9377) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HLA-C at approximately 41 kDa. The expected band size for HLA-C is at 41 kDa.
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