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HLF Antibody

LS-C332384
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetHLF
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    HLF Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 23kDa/33kDa, while the observed MW by Western blot was 35kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:1000) The predicted MW is 23kDa/33kDa, while the observed MW by Western blot was 35kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.81 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3131
  • Target name
    HLF
  • Target description
    HLF transcription factor, PAR bZIP family member
  • Target synonyms
    E2A-HLF fusion transcript protein; hepatic leukemia factor; HLF, PAR bZIP transcription factor; PAR bZIP transcription factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HLF using anti-HLF antibody (A01284-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat lung whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse kidney tissue lysates, Lane 9: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLF antigen affinity purified polyclonal antibody (Catalog # A01284-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HLF at approximately 33KD. The expected band size for HLF is at 33KD.
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