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Western Blot Positive WB detected in: Raji whole cell lysate All lanes: HMBS antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 40, 38, 35, 34 kDa Observed band size: 40 kDa
Western Blot Positive WB detected in: Raji whole cell lysate All lanes: HMBS antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 40, 38, 35, 34 kDa Observed band size: 40 kDa
Western Blot Positive WB detected in: Raji whole cell lysate All lanes: HMBS antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 40, 38, 35, 34 kDa Observed band size: 40 kDa

HMBS Antibody

CSB-PA010524LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetHMBS
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Overview

  • Supplier
    Cusabio
  • Product Name
    HMBS Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3145
  • Target name
    HMBS
  • Target description
    hydroxymethylbilane synthase
  • Target synonyms
    ENCEP, LENCEP, PBG-D, PBGD, PORC, UPS, porphobilinogen deaminase, porphyria, acute; Chester type, pre-uroporphyrinogen synthase, uroporphyrinogen I synthase, uroporphyrinogen I synthetase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08397
  • Protein Name
    Porphobilinogen deaminase
  • Scientific Description
    Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HMBS using anti-HMBS antibody (A01506-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human 22RV1 whole cell lysates, Lane 5: human U20S whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMBS antigen affinity purified polyclonal antibody (Catalog # A01506-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HMBS at approximately 45KD. The expected band size for HMBS is at 39KD.
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