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HMBS / PBGD Antibody (N-Terminus, Biotin)

LS-C458589
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetHMBS
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    HMBS / PBGD Antibody (N-Terminus, Biotin)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Western Blot
  • Applications Supplier
    WB The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Biotin
  • Estimated Purity
    ...
  • Gene ID3145
  • Target name
    HMBS
  • Target description
    hydroxymethylbilane synthase
  • Target synonyms
    PBGD; PBG-D; PORC; porphobilinogen deaminase; porphyria, acute; Chester type; pre-uroporphyrinogen synthase; UPS; uroporphyrinogen I synthase; uroporphyrinogen I synthetase
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HMBS using anti-HMBS antibody (A01506-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human 22RV1 whole cell lysates, Lane 5: human U20S whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMBS antigen affinity purified polyclonal antibody (Catalog # A01506-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HMBS at approximately 45KD. The expected band size for HMBS is at 39KD.
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