WB analysis of various sample lysates using GTX85600 HMG14 (phospho Ser20/Ser24) antibody. Recombinant native and mutant HMGN proteins were treated with kinase PKCalpha to specifically phosphorylate S20 and S24 residues. Lane 1 : HMGN1, non-phosphorylated Lane 2 : HMGN1, phosphorylated Lane 3 : HMGN1 delta20E, delta24E, non-phosphorylated Lane 4 : HMGN1, delta20E, delta24E, phosphorylated Lana 5 : HMGN2, non-phosphorylated Lane 6 : HMGN2, phosphorylated Dilution : 1:1000
WB analysis of various sample lysates using GTX85600 HMG14 (phospho Ser20/Ser24) antibody. Recombinant native and mutant HMGN proteins were treated with kinase PKCalpha to specifically phosphorylate S20 and S24 residues. Lane 1 : HMGN1, non-phosphorylated Lane 2 : HMGN1, phosphorylated Lane 3 : HMGN1 delta20E, delta24E, non-phosphorylated Lane 4 : HMGN1, delta20E, delta24E, phosphorylated Lana 5 : HMGN2, non-phosphorylated Lane 6 : HMGN2, phosphorylated Dilution : 1:1000
HMG14 (phospho Ser20/Ser24) antibody
GTX85600
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetHMGN1
Overview
- SupplierGeneTex
- Product NameHMG14 (phospho Ser20/Ser24) antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:2000. IHC-P: 20 microg/ml. ELISA: 1:5000 - 1:25000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3150
- Target nameHMGN1
- Target descriptionhigh mobility group nucleosome binding domain 1
- Target synonymsHMG14, non-histone chromosomal protein HMG-14, high mobility group nucleosome-binding domain-containing protein 1, high-mobility group (nonhistone chromosomal) protein 14, high-mobility group nucleosome binding 1, nonhistone chromosomal protein HMG-14
- HostRabbit
- IsotypeIgG
- Protein IDP05114
- Protein NameNon-histone chromosomal protein HMG-14
- Scientific DescriptionThe protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMG17, the encoded protein may help maintain an open chromatin configuration around transcribable genes. [provided by RefSeq, Aug 2011]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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