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Western Blot Positive WB detected in: Rat kidney tissue All lanes: HMGCS2 antibody at 4.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57, 53, 51 kDa Observed band size: 57 kDa
Western Blot Positive WB detected in: Rat kidney tissue All lanes: HMGCS2 antibody at 4.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57, 53, 51 kDa Observed band size: 57 kDa
Western Blot Positive WB detected in: Rat kidney tissue All lanes: HMGCS2 antibody at 4.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57, 53, 51 kDa Observed band size: 57 kDa

HMGCS2 Antibody

CSB-PA010567LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetHMGCS2
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Overview

  • Supplier
    Cusabio
  • Product Name
    HMGCS2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3158
  • Target name
    HMGCS2
  • Target description
    3-hydroxy-3-methylglutaryl-CoA synthase 2
  • Target synonyms
    hydroxymethylglutaryl-CoA synthase, mitochondrial, 3-hydroxy-3-methylglutaryl-CoA synthase 2 (mitochondrial), 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial), HMG-CoA synthase, testicular tissue protein Li 88
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP54868
  • Protein Name
    Hydroxymethylglutaryl-CoA synthase, mitochondrial
  • Scientific Description
    This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HMGCS2 using anti-HMGCS2 antibody (A04371-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse RH35 whole cell lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMGCS2 antigen affinity purified polyclonal antibody (Catalog # A04371-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HMGCS2 at approximately 50 kDa. The expected band size for HMGCS2 is at 50 kDa.
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