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ProductsBack/HMW Kininogen antibody [C11C1]
WB analysis of (A) normal human plasma, (B) HMWK-deficient plasma, and (C) purified HMWK proteins using GTX21004 HMW Kininogen antibody [C11C1].
WB analysis of (A) normal human plasma, (B) HMWK-deficient plasma, and (C) purified HMWK proteins using GTX21004 HMW Kininogen antibody [C11C1].
WB analysis of (A) normal human plasma, (B) HMWK-deficient plasma, and (C) purified HMWK proteins using GTX21004 HMW Kininogen antibody [C11C1].

HMW Kininogen antibody [C11C1]

GTX21004
GeneTex
ApplicationsWestern Blot, ELISA
DownloadDatasheet
Product group Antibodies
TargetKNG1
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Overview

  • Supplier
    GeneTex
  • Product Name
    HMW Kininogen antibody [C11C1]
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    C11C1
  • Conjugate
    Unconjugated
  • Gene ID3827
  • Target name
    KNG1
  • Target description
    kininogen 1
  • Target synonyms
    alpha-2-thiol proteinase inhibitor; BDK; BK; bradykinin; fitzgerald factor; HAE6; high molecular weight kininogen; HMWK; kininogen-1; KNG; williams-Fitzgerald-Flaujeac factor
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP01042
  • Protein Name
    Kininogen-1
  • Scientific Description
    This gene uses alternative splicing to generate two different proteins- high molecular weight kininogen (HMWK) and low molecular weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. Bradykinin also functions as an antimicrobial peptide with antibacterial and antifungal activity. In contrast to HMWK, LMWK is not involved in blood coagulation. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Nov 2014]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Kininogen 1/KNG1 using anti-Kininogen 1/KNG1 antibody (PB9278). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kininogen 1/KNG1 antigen affinity purified polyclonal antibody (Catalog # PB9278) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kininogen 1/KNG1 at approximately 72 kDa. The expected band size for Kininogen 1/KNG1 is at 72 kDa.
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