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ProductsBack/HNF1B / HNF1 Beta Antibody (aa50-150)

HNF1B / HNF1 Beta Antibody (aa50-150)

LS-C340538
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetHNF1B
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    HNF1B / HNF1 Beta Antibody (aa50-150)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6928
  • Target name
    HNF1B
  • Target description
    HNF1 homeobox B
  • Target synonyms
    ADTKD3; FJHN; hepatocyte nuclear factor 1-beta; HNF1 beta A; HNF-1B; HNF1beta; HNF-1-beta; HNF2; homeoprotein LFB3; HPC11; LFB3; LF-B3; MODY5; RCAD; T2D; TCF2; TCF-2; transcription factor 2, hepatic; VHNF1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HNF1 beta using anti-HNF1 beta antibody (PB9597). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Liver Tissue Lysate at 50ug, Lane 2: Rat Kidney Tissue Lysate at 50ug, Lane 3: SW620 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HNF1 beta antigen affinity purified polyclonal antibody (Catalog # PB9597) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HNF1 beta at approximately 61 kDa. The expected band size for HNF1 beta is at 61 kDa.
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