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HnRNP-E1 / PCBP1 Antibody (aa188-217, PE)

LS-C226948
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetPCBP1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    HnRNP-E1 / PCBP1 Antibody (aa188-217, PE)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    RPE
  • Estimated Purity
    ...
  • Gene ID5093
  • Target name
    PCBP1
  • Target description
    poly(rC) binding protein 1
  • Target synonyms
    alpha-CP1; epididymis secretory protein Li 85; HEL-S-85; heterogeneous nuclear ribonucleoprotein E1; heterogenous nuclear ribonucleoprotein E1; heterogenous nuclear ribonucleoprotein X; hnRNP-E1; hnRNP-X; HNRPE1; HNRPX; nucleic acid-binding protein SUB2.3; poly(rC)-binding protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PCBP1 using anti-PCBP1 antibody (A02636-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCBP1 antigen affinity purified polyclonal antibody (Catalog # A02636-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCBP1 at approximately 40KD. The expected band size for PCBP1 is at 40KD.
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