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ProductsBack/hnRNP U antibody [3G6]

hnRNP U antibody [3G6]

GTX80385
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
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Product group Antibodies
TargetHNRNPU
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Overview

  • Supplier
    GeneTex
  • Product Name
    hnRNP U antibody [3G6]
  • Delivery Days Customer
    9
  • Application Supplier Note
    Optimal dilutions should be determined experimentally by the researcher.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3G6
  • Conjugate
    Unconjugated
  • Gene ID3192
  • Target name
    HNRNPU
  • Target description
    heterogeneous nuclear ribonucleoprotein U
  • Target synonyms
    DEE54; EIEE54; GRIP120; heterogeneous nuclear ribonucleoprotein U; heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A); hnRNP U; HNRNPU antisense RNA 1; HNRNPU-AS1; HNRPU; nuclear p120 ribonucleoprotein; p120 nuclear protein; pp120; SAFA; SAF-A; U21.1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ00839
  • Protein Name
    Heterogeneous nuclear ribonucleoprotein U
  • Scientific Description
    This gene encodes a member of a family of proteins that bind nucleic acids and function in the formation of ribonucleoprotein complexes in the nucleus with heterogeneous nuclear RNA (hnRNA). The encoded protein has affinity for both RNA and DNA, and binds scaffold-attached region (SAR) DNA. Mutations in this gene have been associated with epileptic encephalopathy, early infantile, 54. A pseudogene of this gene has been identified on chromosome 14. [provided by RefSeq, Jun 2017]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of hnRNP U/p120/HNRNPU using anti-hnRNP U/p120/HNRNPU antibody (A03691-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-hnRNP U/p120/HNRNPU antigen affinity purified polyclonal antibody (Catalog # A03691-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for hnRNP U/p120/HNRNPU at approximately 120 kDa. The expected band size for hnRNP U/p120/HNRNPU is at 90 kDa.
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