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hnRNP U Rabbit pAb

A3917
ABclonal
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHNRNPU
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Overview

  • Supplier
    ABclonal
  • Product Name
    hnRNP U Rabbit pAb
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB,IHC,IF/ICC,IP
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3192
  • Target name
    HNRNPU
  • Target description
    heterogeneous nuclear ribonucleoprotein U
  • Target synonyms
    DEE54, EIEE54, GRIP120, HNRNPU-AS1, HNRPU, SAF-A, SAFA, U21.1, hnRNP U, pp120, heterogeneous nuclear ribonucleoprotein U, HNRNPU antisense RNA 1, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), nuclear p120 ribonucleoprotein, p120 nuclear protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ00839
  • Protein Name
    Heterogeneous nuclear ribonucleoprotein U
  • Reactivity
    Human, Mouse, Rat
  • Reactivity Supplier Note
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of hnRNP U/p120/HNRNPU using anti-hnRNP U/p120/HNRNPU antibody (A03691-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-hnRNP U/p120/HNRNPU antigen affinity purified polyclonal antibody (Catalog # A03691-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for hnRNP U/p120/HNRNPU at approximately 120 kDa. The expected band size for hnRNP U/p120/HNRNPU is at 90 kDa.
Product group Antibodies
Boster Bio
Anti-hnRNP U/p120/HNRNPU Antibody Picoband(r)A03691-3-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetHNRNPU
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Product group Antibodies
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Lane 1:Mouse cerebrum lysates; Lane 2: Rat cerebrum lysates probed with hnRNP U Polyclonal Antibody, Unconjugated (bs-9092R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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ReactivityHuman, Mouse, Rat, Other Species
TargetHNRNPU
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TargetHNRNPU
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Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neurons.
Product group Antibodies
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TargetHNRNPU
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TargetHNRNPU
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Anti-hnRNP U AntibodyCAB3917
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TargetHNRNPU
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