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Western Blot analysis of 293 cells using hnRNP D0 Polyclonal Antibody
Western Blot analysis of 293 cells using hnRNP D0 Polyclonal Antibody
Western Blot analysis of 293 cells using hnRNP D0 Polyclonal Antibody

HNRNPD Antibody

CSB-PA002945
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHNRNPD
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Overview

  • Supplier
    Cusabio
  • Product Name
    HNRNPD Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3184
  • Target name
    HNRNPD
  • Target description
    heterogeneous nuclear ribonucleoprotein D
  • Target synonyms
    AUF1, AUF1A, HNRPD, P37, hnRNPD0, heterogeneous nuclear ribonucleoprotein D0, ARE-binding protein AUFI, type A, AU-rich RNA-binding factor 1, AU-rich element RNA binding protein 1, 37kDa, hnRNP D0
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14103
  • Protein Name
    Heterogeneous nuclear ribonucleoprotein D0
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HNRNPD using anti-HNRNPD antibody (A09982-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human HepG2 whole cell lysates, Lane 7: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HNRNPD antigen affinity purified polyclonal antibody (Catalog # A09982-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for HNRNPD at approximately 37-45KD. The expected band sizes for HNRNPD are at 37, 40, 42 and 45KD.
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