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Western Blot analysis of HuvEc cells using hnRNP L Polyclonal Antibody
Western Blot analysis of HuvEc cells using hnRNP L Polyclonal Antibody
Western Blot analysis of HuvEc cells using hnRNP L Polyclonal Antibody

HNRNPL Antibody

CSB-PA002951
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetHNRNPL
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Overview

  • Supplier
    Cusabio
  • Product Name
    HNRNPL Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3191
  • Target name
    HNRNPL
  • Target description
    heterogeneous nuclear ribonucleoprotein L
  • Target synonyms
    HNRPL, P/OKcl.14, hnRNP-L, heterogeneous nuclear ribonucleoprotein L, epididymis secretory sperm binding protein, hnRNP L
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14866
  • Protein Name
    Heterogeneous nuclear ribonucleoprotein L
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of hnRNP L/HNRNPL using anti-hnRNP L/HNRNPL antibody (A03769-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-hnRNP L/HNRNPL antigen affinity purified polyclonal antibody (Catalog # A03769-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for hnRNP L/HNRNPL at approximately 68 kDa. The expected band size for hnRNP L/HNRNPL is at 64 kDa.
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