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Western blot All lanes: HNRNPM antibody at 2ug/ml + NIH/3T3 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 78, 74 kDa Observed band size: 78 kDa
Western blot All lanes: HNRNPM antibody at 2ug/ml + NIH/3T3 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 78, 74 kDa Observed band size: 78 kDa
Western blot All lanes: HNRNPM antibody at 2ug/ml + NIH/3T3 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 78, 74 kDa Observed band size: 78 kDa

HNRNPM Antibody

CSB-PA010613ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetHNRNPM
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Overview

  • Supplier
    Cusabio
  • Product Name
    HNRNPM Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4670
  • Target name
    HNRNPM
  • Target description
    heterogeneous nuclear ribonucleoprotein M
  • Target synonyms
    CEAR, HNRNPM4, HNRPM, HNRPM4, HTGR1, NAGR1, hnRNP M, heterogeneous nuclear ribonucleoprotein M, CEA receptor, N-acetylglucosamine receptor 1, heterogenous nuclear ribonucleoprotein M4, hnRNA-binding protein M4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP52272
  • Protein Name
    Heterogeneous nuclear ribonucleoprotein M
  • Scientific Description
    Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HNRNPM using anti-HNRNPM antibody (M06017-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HNRNPM antigen affinity purified monoclonal antibody (Catalog # M06017-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HNRNPM at approximately 73 kDa. The expected band size for HNRNPM is at 76 kDa.
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