Horse APLN(Apelin) ELISA Kit

Biorbyt

Horse APLN(Apelin) ELISA Kit

10

standard: 8000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse APLN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse APLN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse APLN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Horse APLN in the samples is then determined by comparing the OD of the samples to the standard curve

125-8000 pg/mL

46 pg/mL

Research Use Only

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apelin(APLN). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Apelin(APLN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apelin(APLN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Apelin(APLN) in the samples is then determined by comparing the OD of the samples to the standard curve.

Equine

41116158